[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"$f1mz6ebwgv4jjy":3},{"data":4,"error":11},{"id":5,"documentId":6,"heading":7,"body":8,"slug":9,"title":10,"title_ar":11,"species":12,"citations":23,"medical_conditions":40,"animal_species":47,"test_discipline":99,"test_method":105,"test_samples":111,"test_purposes":118,"test_settings":149},3930,"x8ui23sobswptt9s6ta6yyg7","تفاعل البوليمراز المتسلسل النوعي لطفيل T. evansi (PCR)","تفاعل البوليمراز المتسلسل (PCR) طريقة جزيئية لكشف طفيل السُّرّة (surra, Trypanosoma evansi infection)، وتذكر المنظمة العالمية لصحة الحيوان (WOAH) أنه في المناطق والمضيفات التي قد تصاب بأنواع أخرى من Trypanozoon مثل T. brucei brucei يمكن الحصول على تأكيد النوع ببادئات أكثر نوعية مثل RoTat 1.2. تذكر دراسة مقارنة أُجريت على الإبل وحيدة السنام في إمارة أبوظبي بالإمارات العربية المتحدة (PMC8959682) أن الطرق الجزيئية تكشف الطفيل في مرحلة مبكرة من العدوى، وبتركيزات منخفضة في العدوى تحت السريرية، بل حتى لدى الحيوانات الحاملة السليمة.\n\n## ما الذي يكشفه\n\nتُستعمل بادئات EVAB للسلالات التي لا تحمل RoTat 1.2، وإذا اشتُبه بأنواع من تحت أجناس أخرى أمكن استعمال بادئات الفاصل الداخلي المنسوخ 1 (ITS1) التي تكشف عدة تحت أجناس وأنواع في تفاعل واحد، لكن بحساسية محدودة.\n\n## تفسير النتيجة وحدوده\n\nإذا اشتُبه بـT. equiperdum، توجب المنظمة النظر في معلومات وبائية أخرى تبرز فيها العلامات السريرية وطريقة الانتقال، إذ لا يوجد حتى الآن اختبار جزيئي بسيط وموثوق يفرّق بين الأنواع الثلاثة T. evansi وT. equiperdum وT. brucei.\n\n## مقارنة بالاختبارات الأخرى\n\nفي دراسة أبوظبي، جاءت نتيجة PCR الآني (real-time PCR) إيجابية في 60% من العينات (46 من 77)، مقابل 18% بالفحص المجهري و22% باختبار التراص على البطاقة (CATT). كانت كل العينات الإيجابية بالمجهر إيجابية أيضًا بـPCR الآني، وبلغت الحساسية النسبية للمجهر 30.4% ولـCATT نحو 37.0% مقارنة به. يخلص باحثو الدراسة إلى أنه لا توجد طريقة حساسة ونوعية بنسبة 100%، وأن إدراج التقنيات الجزيئية الحساسة في خوارزمية الفحص مهم لتشخيص دقيق لداء المثقبيات في الإبل وحيدة السنام.","trypanosoma-evansi-pcr","Trypanosoma evansi PCR",null,[13,14,15,16,17,18,19,20,21,22],"camel","horse","cattle","dog","buffalo","cat","sheep","goat","pig","deer",[24,30,37],{"source":25,"licence":26,"source_url":27,"harvested_by":28,"retrieved_at":29},"Prevalence and animal level risk factors associated with Trypanosoma evansi infection in dromedary camels","test names only (facts); sources cited","https:\u002F\u002Fdoi.org\u002F10.1038\u002Fs41598-022-12817-x","load-reference-corpus.cjs","2026-09-16",{"claims":31,"source":32,"licence":33,"source_url":34,"harvested_by":35,"retrieved_at":36},"Arabic article (body)","WOAH Terrestrial Manual, chapter 3.1.22 Surra in all species (Trypanosoma evansi infection)","facts cited; the Arabic article is original text","https:\u002F\u002Fwww.woah.org\u002Ffileadmin\u002FHome\u002Feng\u002FHealth_standards\u002Ftahm\u002F3.01.22_SURRA_TRYPANO.pdf","apply-chain-articles.cjs","2026-09-30",{"claims":31,"source":38,"licence":33,"source_url":39,"harvested_by":35,"retrieved_at":36},"Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE (PMC8959682)","https:\u002F\u002Fpmc.ncbi.nlm.nih.gov\u002Farticles\u002FPMC8959682\u002F",[41],{"id":42,"documentId":43,"slug":44,"title":45,"heading":46},296,"lpv1ftk9d7cbtt61y51sg0qo","infection-with-trypanosoma-evansi-surra","Infection with Trypanosoma evansi (Surra)","داء السَّرَّا",[48,53,58,63,68,73,78,83,89,94],{"id":49,"documentId":50,"slug":15,"name_en":51,"name_ar":11,"heading":52},1514,"h9lqv8yrfatdtq0hbnxk1jug","Cattle","الأبقار",{"id":54,"documentId":55,"slug":17,"name_en":56,"name_ar":11,"heading":57},1527,"fkk97am9k1b8ygyqodz48cp4","Buffalo","الجاموس",{"id":59,"documentId":60,"slug":13,"name_en":61,"name_ar":11,"heading":62},1520,"jkfqato7h2oc2847wygzmagk","Camel","الجمل العربي",{"id":64,"documentId":65,"slug":16,"name_en":66,"name_ar":11,"heading":67},1518,"rvkmhyozs6s1tlfu4ehuopce","Dog","الكلب",{"id":69,"documentId":70,"slug":20,"name_en":71,"name_ar":11,"heading":72},1515,"kb5w3rwizs3rqlt7he1sz639","Goat","الماعز",{"id":74,"documentId":75,"slug":22,"name_en":76,"name_ar":11,"heading":77},1532,"wrmz17ef7py63cmr2lj39wil","Deer","الأيائل",{"id":79,"documentId":80,"slug":14,"name_en":81,"name_ar":11,"heading":82},1513,"gk2ehqoeeinarnk4yz37j5o4","Horse","الحصان",{"id":84,"documentId":85,"slug":86,"name_en":87,"name_ar":11,"heading":88},1519,"j7o7tp1w2784juwb4te34z5r","domestic-cat","Domestic cat","القط المنزلي",{"id":90,"documentId":91,"slug":19,"name_en":92,"name_ar":11,"heading":93},1516,"t5n3sypt319doki27xt9jx73","Sheep","الأغنام",{"id":95,"documentId":96,"slug":21,"name_en":97,"name_ar":11,"heading":98},1521,"ayia064fhhbaxsj04wpz72xv","Pig","الخنزير",{"id":100,"documentId":101,"key":102,"slug":102,"name_en":103,"name_ar":104},29,"tfi4p3qd9ly8127q00z3ei4k","molecular-diagnostics","Molecular diagnostics (PCR and sequencing)","علم التشخيص الجزيئي",{"id":106,"documentId":107,"key":108,"slug":108,"name_en":109,"name_ar":110},338,"y1mh7e0losm7ibhnqf8qjt9q","nucleic-acid-amplification","Nucleic acid amplification tests","تقنية تضخيم الحمض النووي",[112],{"id":113,"documentId":114,"key":115,"slug":115,"name_en":116,"name_ar":117},206,"a3iu3snjstveko01qcwojdpe","blood","Blood and blood products","دم",[119,125,131,137,143],{"id":120,"documentId":121,"key":122,"slug":122,"name_en":123,"name_ar":124},16,"fxqn1b096lusx9b30r3cwtpm","diagnosis","Diagnosing illness","تشخيص",{"id":126,"documentId":127,"key":128,"slug":128,"name_en":129,"name_ar":130},25,"vfyjqjvu0nd6t9j2pezte9so","surveillance-control","Herd health, surveillance and disease control","صحة القطيع والترصد ومكافحة الأمراض",{"id":132,"documentId":133,"key":134,"slug":134,"name_en":135,"name_ar":136},18,"kuzdtct62xcm37c1505zs6yc","freedom-from-infection","Proving freedom from infection","إثبات الخلو من العدوى",{"id":138,"documentId":139,"key":140,"slug":140,"name_en":141,"name_ar":142},20,"fb37ahgy7q7bvmm44mdlxwyd","movement-trade","Travel, trade and purchase","السفر والتجارة والشراء",{"id":144,"documentId":145,"key":146,"slug":146,"name_en":147,"name_ar":148},15,"fkbpulxyvfkkimk54wx6ctoc","confirmation","Confirming a diagnosis","تأكيد التشخيص",[150],{"id":151,"documentId":152,"key":153,"slug":153,"name_en":154,"name_ar":155},21,"z4e1dt4mwvd2ldm8fvhn2jmn","commercial-reference-laboratory","Commercial reference laboratory","مختبر مرجعي تجاري"]